Journal: bioRxiv
Article Title: Human alveolar Type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells during alveolar repair
doi: 10.1101/2020.06.06.136713
Figure Lengend Snippet: (A) IPA upstream regulator analysis of hAEC2s co-cultured with AHLM. (B) Bulk RNA-seq analysis of MRC5 and AHLM with differential gene expression analysis. (C) Treatment of hAEC2s+AHLM organoids with BMP4 increases SFTPC and decreases KRT5 mRNA expression at D14 of culture. (D) BMP treatment increases SFTPC+ cells and decreases KRT5+ cells in D14 organoids. (E) hAEC2s from IPF lungs demonstrates decreased SFTPC and HHIP expression when compared to hAEC2s from normal donors. (F) Treatment of hAEC2s+AHLM organoids with HHIP increases SFTPC and decreases KRT5 mRNA expression at D14 of culture. (G) HHIP increases SFTPC+ cells and decreases KRT5+ cells in D14 organoids. (H) HHIP-treated AHLM isolated from D14 organoid culture have decreased GLI1 and increased BMP3 and BMP4 mRNA levels. (I) HHIP-treated hAEC2s+AHLM organoids at D14 show significantly higher fraction of pSMAD1/5/8+ cells. See also
Article Snippet: Where applicable, recombinant BMP4 (Cat#314-BP-010; R&D Systems; used 50 ng/mL) and HHIP (Cat#9280-HP-050; R&D Systems; used 5 μg/mL) were added to the media after 48 hours and replenished in every media change.
Techniques: Cell Culture, RNA Sequencing, Gene Expression, Expressing, Isolation